CD62L Mouse anti-Human, Brilliant Violet 421, Clone: SK11 (also known as Anti-Leu-8), BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 743207

Catalog No. BDB743207

Description

The SK11 monoclonal antibody specifically binds to CD62L which is also known as, L-selectin, Leukocyte-endothelial cell adhesion molecule 1 (LECAM1/LECAM-1), Leukocyte adhesion molecule 1 (LAM1/LAM-1), Lymph node homing receptor (LNHR), Leu-8, or TQ1. CD62L is an ñ80 kDa type I transmembrane glycoprotein that belongs to the Selectin/LECAM family. CD62L is differentially expressed on T cells, B cells, monocytes, granulocytes, and a subset of NK cells. The CD62L molecule is the human homolog of the mouse lymph node homing receptor, MEL-14. CD62L plays a role in leucocyte binding to inflamed endothelium and extravasation, as well as mediating lymphocyte homing into peripheral lymphoid tissues through high endothelial postcapillary venules. Soluble CD62L can result from the proteolytic cleavage of cell surface CD62L during cellular activation or inflammation.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: CD62L
• Clone: SK11 (also known as Anti-Leu-8)
• Conjugate: Brilliant Violet 421
• Gene Alias: L-selectin, LECAM-1, SELL, LNHR, LSEL, LAM-1, LEU8, PLNHR, TQ-1, MEL-14
• Immunogen: Human Peripheral Blood T Lymphocytes
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG2a κ