Invitrogen™ CD63 Monoclonal Antibody (M034)

Catalog No.	Quantity
PIMA547655	100 μL

Catalog No. PIMA547655 Supplier Invitrogen™ Supplier No. MA547655

Description

Mouse Monoclonal Antibody

Clone M034 mouse monoclonal antibody detects a 30-60 kDa* protein on SDS-PAGE 'Native' immunoblots of human A549, MeWo, MDA-MB-231, and MCF7 cells, as well as human breast, lung, and skin tissues. This antibody does not detect denatured CD63. The antibody works for western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.

CD63, also known as LAMP-3, is a glycoprotein belonging to the tetraspanin family, primarily located in late endosomes, lysosomes, and secretory vesicles across various cell types. It is expressed intracellularly in resting platelets and basophils, but upon activation, CD63 translocates to the plasma membrane, making it a widely used marker for basophil activation. Unlike basophils, mast cells can expose CD63 without activation. CD63 is expressed on activated platelets, monocytes, macrophages, and granulocytes, as well as endothelial cells, fibroblasts, and smooth muscle cells. It interacts with integrins (such as VLA-3 and VLA-6) and TIMP-1, playing roles in mediating allergic responses, phagocytosis, and cell migration. Additionally, CD63 is involved in the regulation of H/K-ATPase trafficking and ROMK1 channels, and serves as a T-cell costimulation molecule. CD63 expression can be used to predict prognosis in early-stage carcinomas and is identical to the melanoma-associated antigen ME491 and the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome, highlighting its significance in both immune responses and disease processes.

Specifications

• Antigen: CD63
• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: M034
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: CD63
• Gene Accession No.: P08962
• Gene Alias: C75951; CD 63; Cd63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Cd63 molecule; CD63 protein; granulophysin; LAMP-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; mast cell antigen AD1; ME491; melanoma 1 antigen; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; TSPAN30; tspan-30
• Gene Symbols: CD63
• Host Species: Mouse
• Immunogen: Clone (M034) was generated from a proprietary antigen related to the native human CD63 expressed in MeWo melanoma cell line.
• Purification Method: Protein G
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 967
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1