Invitrogen™ CD68 Monoclonal Antibody (ED1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA516654

Catalog No. PIMA516654

Description

This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Mouse anti rat CD68, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of approximately 90 -110 KDa, also known as rat CD68 (Dijkstra et al. 1985).

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

Specifications

• Antigen: CD68
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: 0
• Gene Symbols: Cd68
• Immunogen: Rat spleen cells.
• Quantity: 250 μg
• Primary or Secondary: Primary
• Target Species: Rat, Bovine
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: ED1
• Conjugate: Unconjugated
• Gene: Cd68
• Gene Alias: CD68; CD68 antigen; CD68 molecule; GP110; Lamp4; macrophage antigen CD68; macrosialin; Scard1; scavenger receptor class D, member 1; similar to CD68 antigen
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 287435, 504960
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid