CD69 Hamster anti-Mouse, FITC, Clone: H1.2F3, BD

Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB561929	BD Biosciences 561929	25 μg
BDB557392	BD Biosciences 557392	0.1 mg
BDB553236	BD Biosciences 553236	0.5 mg

Description

The H1.2F3 monoclonal antibody specifically binds to CD69 (Very Early Activation antigen), an 85 kDa disulfide-linked homodimer of differentially glycosylated subunits. CD69 is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells), neutrophils, and macrophages. CD69 is expressed also on thymocytes that are undergoing positive selection; its role in that process is unclear. H1.2F3 mAb augments PMA-induced T-cell stimulation and IFN-γ-induced macrophage stimulation. IL-2-activated NK cells express CD69, and H1.2F3 mAb induces redirected lysis of FcR-bearing target cells by NK cells.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD69
• Concentration: 0.5mg/mL
• Classification: Monoclonal
• Host Species: Hamster
• Target Species: Mouse
• Immunogen: Mouse Dendritic Epidermal T Cell Line Y245
• Purification Method: Affinity Purified
• Description: VEA; Very Early Activation Antigen; AIM; Activation Induced Molecule

• Clone: H1.2F3
• Applications: Flow Cytometry
• Conjugate: FITC
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.