CD69 Mouse anti-Human, RB545, Clone: FN50 (also known as FN 50), BD Horizon™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 569273

Catalog No. BDB569273

Description

The FN50 monoclonal antibody specifically binds to human CD69. CD69 is also known as activation-induced molecule (AIM), early activation antigen (EA-1), very early activation antigen (VEA), C-type lectin domain family 2 member C (CLEC2C), MLR-3, GP32/28 and Leu-23. CD69 is a transmembrane type II homodimer receptor. CD69 is comprised of disulfide-linked, differentially glycosylated core protein subunits that are approximately 28 and 34 kDa in size. Each subunit contains a C-type lectin domain. CD69 is expressed on activated T, B, and natural killer (NK) lymphocytes, thymocytes, neutrophils, eosinophils and platelets. In normal peripheral blood, a small and variable percentage of lymphocytes typically express detectable membrane CD69 antigen. Upon activation, CD69 antigen expression increases on lymphocytes. Peak CD69 expression generally occurs within 18 hours of activation, preceding the appearance of HLA-DR, IL-2Rα (CD25) and transferrin receptor (CD71). CD69 is highly expressed on the bright CD3+ subset of thymocytes. FN50 monoclonal antibody labels NK cells and most lymphocytes of the follicular mantle and perifollicular/interfollicular zone as well as germinal center T cells of lymph nodes and tonsils. Studies indicate that CD69 serves as a signaling receptor in the activation of a variety of cell types.

CD69 Monoclonal antibody specifically detects CD69 in Human samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD69
• Classification: Monoclonal
• Conjugate: RB545
• Gene Alias: AIM; CLEC2C; EA1; GP32/28; Leu23; MLR-3; VEA; BL-AC/P26
• Immunogen: Anti-μ stimulated human B lymphocytes
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified
• Excitation/Emission: Excitation Source: Blue 488 nm, Excitation Max: 496 nm, Emission Max: 545 nm

• Applications: Flow Cytometry
• Clone: FN50 (also known as FN 50)
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 969
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
• Isotype: IgG1 κ