Invitrogen™ CD7 Polyclonal Antibody

Catalog No.	Quantity
PIPA532332	500 μL

Catalog No. PIPA532332 Supplier Invitrogen™ Supplier No. PA532332

Description

Rabbit Polyclonal Antibody

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

CD7, also known as gp40 or Leu9, is a 40 kDa receptor and member of the immunoglobulin gene superfamily. It features an N-terminal region (amino acids 1-107) that is highly homologous to Ig kappa light chains, while its carboxyl-terminal region is proline-rich, forming a stalk from which the Ig domain projects. CD7 is prominently expressed on the majority of immature and mature T lymphocytes, as well as T cell leukemias. It is also found on natural killer cells, a small subpopulation of normal B cells, and malignant B cells. CD7 plays a crucial role in modulating immune cell activity. Cross-linking of surface CD7 enhances T cell and NK cell functions, as evidenced by increased calcium flux, expression of adhesion molecules, cytokine secretion, and proliferation. CD7 directly associates with phosphoinositol 3-kinase, and its ligation induces the production of D-3 phosphoinositides and tyrosine phosphorylation. The expression of CD7 is an important marker in leukemia diagnostics, highlighting its significance in both normal immune function and disease states.

Specifications

• Antigen: CD7
• Applications: Immunohistochemistry (Paraffin)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene: CD7
• Gene Accession No.: P09564
• Gene Alias: Cd7; CD7 antigen; CD7 antigen (p41); Cd7 molecule; GP40; LEU-9; p41 protein; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9; Tp40; TP41
• Gene Symbols: CD7
• Host Species: Rabbit
• Immunogen: Synthetic peptide derived from internal region of human CD7 protein.
• Purification Method: Antigen affinity chromatography
• Quantity: 500 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 924
• Target Species: Human
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG