Invitrogen™ CD72 Monoclonal Antibody (Bu40)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA516599

Catalog No. PIMA516599

Description

This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.

CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.

Specifications

• Antigen: CD72
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P21854
• Gene Symbols: CD72
• Immunogen: The details of the immunogen for this antibody are not currently available.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: Bu40
• Conjugate: Unconjugated
• Gene: CD72
• Gene Alias: B-cell differentiation antigen CD72; CD72; CD72 antigen; Cd72 molecule; CD72b; CD72c; Ly-19; Ly32; Ly-32; LYB2; Lyb-2; Ly-m19; lymphocyte antigen 32; RP11-331F9.9
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 971
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid