CD72 Mouse anti-Human, Brilliant Violet 711, Clone: J4-117, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 743798

Catalog No. BDB743798

Description

The J4-117 monoclonal antibody specifically recognizes CD72. CD72 is a 39-43 kDa type II integral membrane protein that belongs to the C-type lectin superfamily. CD72 is expressed on B cells from early precursor stages through mature B cells. CD72 is not expressed on plasma cells. CD72 is also expressed on follicular dendritic reticulum cells and some macrophages. CD72 has been reported to bind to CD5, a glycoprotein expressed on all mature T cells and a subpopulation of B cells, suggesting a role in regulating T- and B-cell activation and proliferation.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.

Specifications

• Antigen: CD72
• Clone: J4-117
• Conjugate: Brilliant Violet 711
• Gene Alias: Lyb-2, LYB2
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG2b κ

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.