Invitrogen™ CD73 Monoclonal Antibody (A6G2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA550558

Catalog No. FEMA550558

Description

Positive control: U87MG cell lysates, NCI-H441 xenograft tissue, U87MG xenograft tissue, U87-MG. Predicted band size: 63 kDa Subcellular Location: Cell membrane.

CD73, also known as Ecto-5-prime-nucleotidase or 5-prime-ribonucleotide phosphohydrolase, is an enzyme that catalyzes the conversion of purine 5-prime mononucleotides to nucleosides at neutral pH, with AMP as its preferred substrate. It is composed of a dimer of two identical 70 kDa subunits, externally bound to the plasma membrane via a glycosyl phosphatidyl inositol linkage. CD73 serves as a marker of lymphocyte differentiation and is expressed on a subset of lymphocytes, increasing during lymphocyte maturation. It is found on memory CD4 T cells, which resemble uncommitted primed precursor helper cells (Thpp) capable of differentiating into Th1 or Th2 cells, and is also present on regulatory T cells. CD73 deficiency is associated with various immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes, distinguishable from CD73 by their substrate affinities, requirement for divalent magnesium ions, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene is localized to chromosome 6q14-q21, and defects in this gene can lead to conditions such as calcification of joints and arteries, and intestinal tuberculosis. Additionally, two transcript variants encoding different isoforms of CD73 have been identified.

Specifications

• Antigen: CD73
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P21589
• Gene Symbols: NT5E
• Immunogen: Recombinant protein within Human CD73 27-549/574.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: A6G2
• Conjugate: Unconjugated
• Gene: NT5E
• Gene Alias: 2210401F01Rik; 5 nucleotidase, ecto; 5' nucleotidase, ecto; 5'-NT; 5'-nucleotidase; 5'-nucleotidase ecto; 5'-nucleotidase, ecto (CD73); AI447961; CALJA; CD73; E5NT; ecto-5'-nucleotidase; eN; eNT; NT; Nt5; Nt5e; NTE; Purine 5-Prime-Nucleotidase
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 4907
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid