CD74 Mouse anti-Human, Alexa Fluor™ 594, Clone: BU45, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234568AF594

Catalog No. NP23468A94

Description

CD74 Monoclonal specifically detects CD74 in Human samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Specifications

• Antigen: CD74
• Classification: Monoclonal
• Conjugate: Alexa Fluor 594
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: CD74
• Immunogen: B lymphoblastoid cell line HFB1
• Quantity: 0.1 mL
• Gene ID (Entrez): 972
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
• Clone: BU45
• Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen
• Gene Alias: CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated), CD74 molecule, major histocompatibility complex, class II invariant chain, DHLAGgamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLADG, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia antigen-associated invariant chain, Ia-associated invariant chain, Ia-GAMMA, Ii, MHC HLA-DR gamma chain, p33
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Primary or Secondary: Primary
• Test Specificity: Recognizes proteins of 33, 35 and 41kDa, which are identified as various isoforms of CD74. Its epitope is localized in the extracellular domain of CD74. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The CD74 molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, and dendritic cells and by various types of carcinomas. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Content And Storage: Store at 4°C in the dark.