CD74 Mouse anti-Human, Brilliant Violet 605, Clone: LN2 (also known as LN-2), BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 743733

Catalog No. BDB743733

Description

The LN2 monoclonal antibody specifically recognizes CD74, the cell surface MHC class II-associated invariant chain molecule (41, 35, 33 kDa). CD74 is moderately expressed on B cells, and weakly on monocytes and macrophages. Other reactive cells include Langerhans cells, dendritic cells, a subset of activated T cells and thymic epithelium. The LN2 monoclonal antibody labels a nuclear membrane protein of follicular center and mantle zone B cells and a cytoplasmic protein of cells which bind HLA class II antibodies. CD74 is postulated to play a role in antigen processing and presentation through intracellular transport and expression of class II molecules on the cell surface. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections without citrate pretreatment.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.

Specifications

• Antigen: CD74
• Clone: LN2 (also known as LN-2)
• Conjugate: Brilliant Violet 605
• Gene Alias: DHLAG, HLADG, HLA-DR-gamma, HG2A, Ia-GAMMA
• Immunogen: Nuclei from Human SU-DHL-4 Lymphoma Cell Line
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ