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CD75/CD75s Mouse anti-Human, Alexa Fluor 647, Clone: ZB55, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 566350

Catalog No. BDB566350

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The ZB55 monoclonal antibody specifically recognizes the carbohydrate CD75 (lactosamines) and CD75s (sialylated lactosamines) antigens which are expressed in association with various glycolipids and glycoproteins. CD75 is expressed on mature B cells and germinal center B lymphocytes whereas CD75s is expressed on mature B cells. CD75 expression is lost on B cells differentiating to plasma cells whereas CD75s expression is lost on both germinal center B cells and B cells differentiating into plasma cells. CD75 and CD75s may be expressed by some T cells and can be useful markers for distinguishing different types of malignant B cells.

Alexa Fluor™ 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor™ 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor™ 647 cannot be used simultaneously.


Alexa Fluor 647
Affinity Purified
Mouse IgM, κ
100 Tests
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
CD75/CD75s, CDw75
Human CD32 Peptides
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor™ 647 under optimum conditions, and unreacted Alexa Fluor™ 647 was removed.
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