Invitrogen™ CD75 Monoclonal Antibody (LN-1), eFluor™ 660, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 50075942

Catalog No. 50-075-942

Description

Description: This LN-1 monoclonal antibody reacts with human CD75, a cell surface carbohydrate determinant produced by alpha-2,6-sialytransferase. CD75 is expressed predominantly on mature B cells in peripheral blood and germinal centers, as well as several B cell lines (e.g., Raji, Ramos). CD75 is also expressed on non-immune tissues such as breast, prostate, colon, liver, and kidney. Reports demonstrate that CD75 is a ligand of CD22. Studies have shown that, depending on fixation and tissue processing, the LN-1 antibody also recognizes astrocytes and microglia in the human brain. Applications Reported: This LN-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This LN-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene.

Specifications

• Antigen: CD75
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P15907
• Gene Symbols: ST6GAL1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 6480
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: LN-1
• Conjugate: eFluor 660
• Gene: ST6GAL1
• Gene Alias: Alpha 2,6-ST 1; AW742324; B-cell antigen CD75; beta galactoside alpha 2,6 sialyltransferase 1; beta-galactoside alpha-2,6-sialyltransferase 1; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; Sialyltransferase 1; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); Sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase); Sialyltransferase 1 (beta-galactoside alpha-26-sialytransferase); SIAT1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6 beta-galactoside alpha-2,6-sialyltransferase 1; ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1; St6gal; ST6Gal I; St6gal1; ST6GalI; St6Gal-I; ST6N
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgM