CD8 alpha Monoclonal Antibody (53-6.7), Invitrogen™

Rat Monoclonal Antibody

Supplier:  Thermo Scientific MA184018

Catalog No. PIMA184018

Description

MA1-84018 is recommended for IHC using acetone-fixed frozen tissue sections. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. This antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Rat anti Mouse CD8 alpha antibody, clone 53-6.7 detects mouse CD8a, also known as Lyt-2.

CD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).

Specifications

• Antigen: CD8 alpha
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Gene: Cd8a
• Gene Alias: CD8, Leu-2, MAL, p32, CD8a
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 12525
• Content And Storage: 4°C or -20°C if preferred
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: 53-6.7
• Conjugate: Unconjugated
• Gene Accession No.: P01731
• Gene Symbols: CD8A
• Immunogen: Murine thymus or spleen.
• Quantity: 250 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a