CD8 Mouse anti-Human, PE-Cy5, Clone: RPA-T8, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB561951	BD Biosciences 561951	25 Tests
BDB555368	BD Biosciences 555368	100 Tests

Description

The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α) . CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8 α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors.

Specifications

• Antigen: CD8
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine5
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Isotype: IgG1 κ
• Purification Method: Affinity Purified
• Description: CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32

• Clone: RPA-T8
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Baboon, Cynomolgus, Human, Rhesus Monkey
• Immunogen: Human CD8a
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.