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CD8 Mouse anti-Human, RB545, Clone: RPA-T8, BD Horizon™

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BDB569241 25 Tests
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Catalog No. BDB569241 Supplier BD Biosciences Supplier No. 569241
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Description

Mouse Monoclonal Antibody

The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8a). CD8a is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8a is expressed by the majority of thymocytes, by subpopulations of aβ T cells and γδ T cells and by some NK cells. Cell surface CD8a is expressed either as a disulfide-linked homodimer (CD8aa) or as a heterodimer (CD8aβ) when disulfide-bonded to a CD8 beta chain (CD8β). CD8-positive aβ T cells coexpress both CD8aa homodimers and CD8aβ heterodimers whereas some γδ T cells and NK cells express CD8aa homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8a binds to a non-polymorphic determinant on HLA class I molecules (a3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8a associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors.

CD8 Monoclonal antibody specifically detects CD8 in Human samples. It is validated for Flow Cytometry
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD8
Applications Flow Cytometry
Classification Monoclonal
Clone RPA-T8
Conjugate RB545
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Gene Alias CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
Host Species Mouse
Immunogen Human CD8a
Purification Method Affinity Purified
Quantity 25 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 925
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.
Form Purified
Isotype IgG1 κ
Excitation/Emission Excitation Source: Blue 488 nm, Excitation Max: 496 nm, Emission Max: 545 nm
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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