Invitrogen™ CD83 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA596044

Catalog No. PIPA596044

Description

Positive Samples: Jurkat, Mouse intestine; Cellular Location: Membrane, Single-pass type I membrane protein Immunogen sequence: TPEVKVACSE DVDLPCTAPW DPQVPYTVSW VKLLEGGEER METPQEDHLR GQHYHQKGQN GSFDAPNERP YSLKIRNTTS CNSGTYRCTL QDPDGQRNLS GKVILRVTGC PAQRKEETFK KYRA.

CD83 cell surface antigen is a 40-45kD glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA. In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein and this molecule is a member of the immunoglobulin superfamily that is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules. Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.

Specifications

• Antigen: CD83
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CD83
• Gene Alias: B-cell activation protein; BL11; CD antigen CD83; CD83; CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; cell surface protein HB15; cell-surface glycoprotein; HB15; hCD83; mCD83
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12522, 361226, 9308
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.6 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O88324, Q01151
• Gene Symbols: CD83
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-143 of human CD83 (NP_0042241).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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