Invitrogen™ CD84 Monoclonal Antibody (2G7), Brilliant Ultra Violet™ 737, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
36-708-4941	Invitrogen™ 367084941	25 Tests
36-708-4942	Invitrogen™ 367084942	100 Tests

Description

Description The monoclonal antibody 2G7 reacts to CD84, a transmembrane protein of the Ig SLAM superfamily. Expression of CD84 is found on mature B cells, a subset of T cells (mainly CD4+CD45RA+), monocytes and platelets. CD84 is found on a percentage of human hematopoietic progenitor (CD34+) cells. Signaling through CD84 via homotypic interactions involves phosphorylation and recruitment of SAP and EAT-2 leading to production of IFN gamma and T cell activation. Applications Tested This 2G7 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Blocking Buffers When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401</a>) or Brilliant Stain Buffer (Product # 00-4409-75</a>) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Light sensitivity This tandem dye is sensitive to photo-i...

CD84 is expressed on mature B cells and on B cell lines, including pre B cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.

Specifications

• Antigen: CD84
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: Brilliant Ultraviolet 737
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9UIB8
• Gene ID (Entrez): 8832
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 2G7
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Human
• Gene: CD84
• Gene Alias: A130013D22Rik; CD84; CD84 antigen; CD84 antigen (leukocyte antigen); CD84 molecule; CDw84; cell surface antigen MAX.3; DKFZp781E2378; hCD84; Hly9-beta; leucocyte differentiation antigen CD84; leukocyte antigen CD84; leukocyte differentiation antigen CD84; LY9B; mCD84; RGD1564553; Signaling lymphocytic activation molecule 5; SLAM family member 5; SLAMF5
• Gene Symbols: CD84
• Product Type: Antibody