Invitrogen™ CD85j (ILT2) Monoclonal Antibody (GHI/75)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA544079

Catalog No. PIMA544079

Description

This antibody recognizes an extracellular epitope of CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual). Functional application: Blocking of HLA-G induced TGF-beta1 production.

CD85j, also known as a member of the leukocyte immunoglobulin-like receptor (LIR) family, is a single transmembrane glycoprotein with a long cytoplasmic domain containing four immunoreceptor tyrosine-based inhibitory motifs (ITIMs). These motifs signal through interactions with SHP-1, transducing negative signals that inhibit immune response stimulation. CD85j is expressed on myeloid cells such as monocytes and dendritic cells, as well as on subsets of lymphoid cells including NK, T, and B cells. The expression of CD85j is correlated with leukemias such as acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL). On CD8+ cells, CD85j expression is associated with effector cell function and plays a significant role in viral infections, including HIV, Epstein-Barr virus, and cytomegalovirus (CMV). CD85j binds to MHC class I molecules, including HLA-G, A, F, B27, E, and F, on antigen-presenting cells. The receptor helps control inflammatory responses and cytotoxicity, focusing the immune response and limiting autoreactivity. CD85j belongs to the subfamily B class of LIR receptors, which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and multiple transcript variants encoding different isoforms have been identified for this gene. Its gene is located in a cluster at chromosomal region 19q13.4.

Specifications

• Antigen: CD85j (ILT2)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.4
• Gene Accession No.: Q8NHL6
• Gene Symbols: LILRB1
• Immunogen: Hairy cell leukaemia cells.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Clone: GHI/75
• Conjugate: Unconjugated
• Gene: LILRB1
• Gene Alias: CD85 antigen-like family member J; CD85j; Ig-like transcript 2; ILT2; ILT-2; Immunoglobulin-like transcript 2; leucocyte Ig-like receptor B1; leukocyte immunoglobulin like receptor B1; leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor subfamily B member 3-like; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1; LILRB1; LIR1; LIR-1; LOC100912456; MIR7; MIR-7; Monocyte/macrophage immunoglobulin-like receptor 7; myeloid inhibitory receptor 7; PIRB; PIR-B
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 10859
• Content And Storage: 4°C, do not freeze
• Form: Liquid