CD88 (C5aR1) Monoclonal Antibody (S5/1), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
25-088-842	Invitrogen™ 25088842	100 Tests

Description

Description: This S5/1 monoclonal antibody recognizes human CD88 also known as C5aR1. C5aR1 is a complement receptor widely expressed on cells of the myeloid lineage. Clone S5/1 has been reported to block C5a binding. Applications Reported: This S5/1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This S5/1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known. C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production. CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex. Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.

Specifications

• Antigen: CD88 (C5aR1)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene ID (Entrez): 728
• Isotype: IgG2a κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: S5/1
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine7
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P21730
• Immunogen: BSA-Conjugated Peptide aa. 1-31.
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: C5AR1