CD88 Mouse anti-Human, Brilliant Violet 605, Clone: D53-1473, BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742317
The D53-1473 monoclonal antibody specifically recognizes CD88, a 40 kDa polypeptide expressed on granulocytes, monocytes and macrophages. CD88 is a G type protein-coupled receptor with seven transmembrane domains. It binds to C5a, theαchain of the complement component C5. C5a is cleaved from C5 during complement activation. The binding of C5a to C5a receptor (C5aR, CD88) on granulocytes leads to various effects including shedding of selectins, upregulation of adhesion molecules, chemotaxis, granule exocytosis and activation of NADPH oxidase. Clone D53-1473 was developed using a known C5aR N-terminal extracellular hydrophilic region (a.a. 1-31) peptide as immunogen. This antibody is useful in inflammation and chemotaxis research.This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|C5a Receptor, C5aR, C5a anaphylatoxin receptor|
|Mouse IgG1, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Brilliant Violet 605|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV605 under optimal conditions that minimize unconjugated dye and antibody.|
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