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CD8a Monoclonal Antibody (AMC908), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
$120.00 - $307.00
Specifications
Antigen | CD8a |
---|---|
Clone | AMC908 |
Concentration | 0.5 mg/mL |
Applications | Immunohistochemistry (Paraffin), Western Blot |
Classification | Monoclonal |
Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
14-000-880
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Invitrogen™
14000880 |
25 μg |
Each for $120.00
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50-112-2627
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Invitrogen™
14000882 |
100 μg |
Each for $307.00
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Description
Description: This AMC908 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Applications Reported: This AMC908 antibody has been reported for use in western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This AMC908 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval. This can be used at less than or equal to 0.5 μg/mL. This AMC908 antibody has been tested by western blot of reduced peripheral blood mononuclear cells and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.Specifications
CD8a | |
0.5 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with 0.09% sodium azide; pH 7.2 | |
925, 926 | |
Primary | |
4°C | |
CD8B |
AMC908 | |
Immunohistochemistry (Paraffin), Western Blot | |
Unconjugated | |
Mouse | |
Human | |
P01732, P10966 | |
IgG2a κ | |
Affinity Chromatography | |
Antibody |
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