Invitrogen™ CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), FITC, eBioscience™

Description

Mouse Monoclonal Antibody

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells. Applications Reported: The HIS51 antibody has been reported for use in flow cytometric analysis. Applications Tested: The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Specifications

• Antigen: CD90.1 (Thy-1.1)
• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Applications: Flow Cytometry
• Cell Type: Primary Rat Astrocytes
• Classification: Animal Origin-free, Monoclonal
• Concentration: 1 X, 0.5 mg/mL
• Clone: HIS51
• Form: Liquid
• Product Type: MEM (Minimum Essential Medium), Antibody
• Conjugate: FITC
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• With Additives: Low Glucose, Phenol Red
• Gene: Thy1
• Without Additives: No Glutamine, No HEPES, No Sodium Pyruvate
• Gene Accession No.: P01830, P01831
• Gene Alias: CD7; CD90; FLJ33325; T25; Thy 1.2; Thy1; Thy-1; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 glycoprotein; thy-1 membrane glycoprotein; Thy-1 protein; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta; Thymus cell surface antigen
• Gene Symbols: Thy1
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Host Species: Mouse
• Product Line: Gibco™
• Quantity: 500 mL, 50 μg
• Shipping Condition: Room Temperature
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 21838, 24832
• Target Species: Mouse, Rat
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!, Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 24 months from date of manufacture
• Isotype: IgG2a κ

Safety and Handling

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