CD90/Thy1 Antibody (AF-9) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247755A	Novus Biologicals NBP2477550.1MG	0.1 mg
NBP247755B	Novus Biologicals NBP2477550.2MG	0.2 mg

Description

CD90/Thy1 Monoclonal specifically detects CD90/Thy1 in Human, Bovine samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: CD90/Thy1
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Hematopoietic Stem Cell Markers, Hepatic Stem Cell Markers, Immunology, Innate Immunity, Mesenchymal Stem Cell Markers, Signal Transduction, Stem Cell Markers
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 7070
• Immunogen: Human T-lymphoma cells
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: AF-9
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Bovine
• Gene Alias: CD90, CD90 antigen, CDw90, FLJ33325, Thy-1 antigen, Thy-1 cell surface antigen, thy-1 membrane glycoprotein, Thy-1 T-cell antigen
• Gene Symbols: THY1
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 18-35kDa, identified as CD90 (also known as Thy1). CD90 is a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kDa) inserted into the cell membrane via a GPI anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40% of CD34+ cells in bone marrow, and <1% of CD3+CD4+ lymphocytes in peripheral circulation. It is also expressed by human lymph node HEV endothelium but not other endothelia. Lastly, it is expressed by a limited number of lymphoblastoid and leukemic cell lines.