CD91 Mouse anti-Human, BUV395, Clone: A2MR-α2, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 743862

Catalog No. BDB743862

Description

The A2MR-α2 monoclonal antibody specifically reacts with a 600 kDa, type I membrane single protein, also known as theα2 Macroglobulin (α2M) receptor/low density lipoprotein receptor-related protein 1 (LRP-1). Reported to be an endocytic receptor involved with intracellular signalling, lipid homeostasis, clearance of apoptotic cells, andα2 Macroglobulin mediated clearance of secreted amyloid precursor protein found in Alzheimer patients. The single chain receptor undergoes cleavage, shortly after synthesis, into the 85 kDa transmembraneβchain that non-covalently binds to the extracellular 500-515 kDa a chain. It has a broad cellular distribution, but in the hematopoietic system it is expressed on monocyte lineage cells.α2M/LRP-1 mediates endocytosis of a variety of ligands includingα2M-proteinase complexes, plasminogen activators in complex with plasminogen activator inhibitor, or Pseudomonas /Exotoxin A. Ligand binding toα2M/LRP-1 is followed by rapid transport of the ligand to lysosomes for degradation.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: CD91
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: A2MR, APOER, APR, IGFBP3R, TGFBR5, LRPA, LRP1
• Immunogen: Purified Human α2 Macroglobulin
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human

• Clone: A2MR-α2
• Conjugate: Brilliant Ultraviolet 395
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse BALB/c IgG₁, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal