Invitrogen™ CD95 (APO-1/Fas) Monoclonal Antibody (SM 1-13), eBioscience™

Catalog No.	Quantity
50-176-97	100 μg

Catalog No. 50-176-97 Supplier Invitrogen™ Supplier No. BMS1030

Description

Mouse Monoclonal Antibody

Description: APO-1/Fas (CD95), a member of the TNF/NGF receptor superfamily, is a glycosylated 48 kDa surface protein containing a single transmembrane region. APO-1 is expressed on a variety of human B and T cell lines, on many different tumor cells and on various normal human tissues. APO-1 also occurs in a soluble form (sAPO-1) devoid of a transmembrane region. Triggering of APO-1 by its ligand or by certain anti-APO-1 monoclonal antibodies results in rapid induction of programmed cell death, apoptosis, in susceptible cells. The tissue distribution of APO-1 and of the APO-1 ligand suggests that the APO-1 receptor/ligand system plays an important role in various aspects of mammalian development and especially in the homeostasis of the immune system. Expression of the APO-1 cell surface protein is enhanced by IFN-gamma and TNF and by activation in lymphocytes. Applications Tested: ELISA, Flow Cytometry.

CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.

Specifications

• Antigen: CD95 (APO-1/Fas)
• Applications: ELISA, Flow Cytometry
• Classification: Monoclonal
• Clone: SM 1-13
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative; pH 7.2-7.4
• Gene: FAS
• Gene Accession No.: P25445
• Gene Alias: AI196731; ALPS1A; APO1; APO-1; Apo1 antigen; Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; APT1; CD95; CD95 antigen; FAS; Fas (TNF receptor superfamily member 6); Fas (TNF receptor superfamily member); Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas antigen (ATP1); Fas cell surface death receptor; Fas receptor; FAS1; FASLG receptor; FASTM; lpr; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; TNFR6; Tnfrsf6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily member 26; Tumor necrosis factor receptor superfamily, member 6
• Gene Symbols: FAS
• Host Species: Mouse
• Immunogen: Rec. Human CD95 (APO-1/Fas).
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 355
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1