Invitrogen™ CD95 (APO-1/Fas) Monoclonal Antibody (SM1/23), FITC, eBioscience™

Catalog No.	Quantity
50-177-96	100 Tests

Catalog No. 50-177-96 Supplier Invitrogen™ Supplier No. BMS140FI

Description

Mouse Monoclonal Antibody

BMS140 is suitable as primary antibody for staining in FACS analysis. BMS140FI can be used for direct staining of cells. BMS140BT can be used in a two-step procedure using the biotin-(strept-)avidin system. Functional studies: BMS140 was found to block induction of apoptosis induced by clone SM1/1 or other commercial anti APO-1 antibodies. Optimal dilutions should be determined by the individual laboratory for each application. Applications Tested: Flow Cytometry. Purity: >95%. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.

Specifications

• Antigen: CD95 (APO-1/Fas)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: SM1/23
• Concentration: 10 μL/Test
• Conjugate: FITC
• Formulation: TBS with 1% BSA and 0.02% sodium azide; pH 7.2-7.4
• Gene: FAS
• Gene Accession No.: P25445
• Gene Alias: AI196731; ALPS1A; APO1; APO-1; Apo1 antigen; Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; APT1; CD95; CD95 antigen; FAS; Fas (TNF receptor superfamily member 6); Fas (TNF receptor superfamily member); Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas antigen (ATP1); Fas cell surface death receptor; Fas receptor; FAS1; FASLG receptor; FASTM; lpr; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; TNFR6; Tnfrsf6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily member 26; Tumor necrosis factor receptor superfamily, member 6
• Gene Symbols: FAS
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 355
• Target Species: Human
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b