CD95 Hamster anti-Mouse, FITC, Clone: JO2, BD

Catalog No.	Quantity
BDB561979	0.1 mg
BDB554257	0.5 mg

Catalog No. BDB561979 Supplier BD Biosciences Supplier No. 561979

Description

Hamster Monoclonal Antibody

Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such asJo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis.

The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb has been reported to immunoprecipitate mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation.

Specifications

• Antigen: CD95
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: JO2
• Concentration: 0.5mg/mL
• Conjugate: FITC
• Description: Apo-1; Apt1; Fas; FASLG receptor; lpr; TNFR6; Tnfrsf6; TNR6
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Hamster
• Immunogen: WR19L mouse lymphoma cells transformed with recombinant mouse Fas
• Purification Method: Affinity Purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2 λ2

For Research Use Only.