CD95 Mouse anti-Human, Brilliant Violet 421, Clone: DX2, BD

Catalog No.	Quantity
BDB566258	25 Tests

Catalog No. BDB566258 Supplier BD Biosciences Supplier No. 566258

Description

Mouse Monoclonal Antibody

The DX2 monoclonal antibody specifically binds to the human Fas antigen (also called APO-1). This 45 kDa transmembrane cell surface molecule was designated as CD95 at the Fifth HLDA Workshop. Fas is a member of the TNF-receptor superfamily and is also known as Tumor necrosis factor receptor superfamily member 6 (TNFRSF6). It is expressed on a variety of normal and neoplastic cells including activated T and B lymphocytes and some undifferentiated thymocytes. The Fas/CD95 antigen is a polypeptide that plays a role in the programmed sequence of events leading to cell death, termed apoptosis. The DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines. Crosslinking CD95 with DX2 antibody delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon™ BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon™ BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: CD95
• Classification: Monoclonal
• Clone: DX2
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Gene Alias: APO-1, FAS, TNFRSF6, APT1, ALPS1A, FAS1, FASTM, FASLG receptor
• Host Species: Mouse
• Immunogen: Human CD95-transfected L Cells
• Purification Method: Affinity Purified
• Quantity: 25 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ