CD99 Monoclonal Antibody (3B2/TA8), Super Bright™ 436, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
62-099-742	Invitrogen™ 62099742	100 Tests

Description

Description: The 3B2/TA8 monoclonal antibody reacts with human CD99, also known as E2 antigen and MIC2. CD99 is an approximately 32 kDa sialoglycoprotein and is expressed by all leukocytes. CD99 is involved in T cell adhesion and activation. Applications Reported: This 3B2/TA8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3B2/TA8 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Specifications

• Antigen: CD99
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P14209
• Gene Symbols: CD99
• Primary or Secondary: Primary
• Product Type: Antibody

• Clone: 3B2/TA8
• Applications: Flow Cytometry
• Conjugate: Super Bright 436
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 4267
• Isotype: IgG2a κ
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: CD99