Cdc2 p34Mouse anti-Human, Mouse, Rat, Clone: POH-1, Rockland™

Catalog No.	Quantity
50-199-0506	100 μg

Catalog No. 50-199-0506 Supplier Rockland Immunochemicals Supplier No. 200301160

Description

Mouse Monoclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.

CDK1 (CDC2, P34CDC2) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDK1 also play important regulatory roles in cell cycle control. Destruction of cyclin B during metaphase results in inactivation of CDK1, allowing mitotic exit and cell division. CDK1 also contributes to the control of DNA replication and can be inhibited by several transcriptional targets of p53, such as p21WAF. The ability of CDK1 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2. Research studies have shown that expression of CDK1 in the brain has been linked to both neurogenesis and apoptosis. In vertebrates, multiple cyclins like CDK1 have been identified, including a total of eight such regulatory proteins in mammals. Alternatively spliced transcript variants encoding different isoforms of CDK1 have been found.

Specifications

• Antigen: Cdc2 p34
• Applications: ELISA, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: POH-1
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.5M NaCl and 0.01% sodium azide; pH 7.2
• Gene: CDK1
• Gene Accession No.: P06493, P11440, P39951
• Gene Alias: 5363; ABL1; BOS_24079; cdc; CDC2; cdc-2; cdc2 kinase; CDC28A; Cdc2a; cdc2-a; cdc2Dm; cdc2x1.1; CDCDm; CDK1; Cdk-1; cdk1.S; CDK1/CDC2; cdk1-a; cdk1-b; Cdk1-PA; CDKN1; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; cell division control; Cell division control protein 2 homolog; Cell division control protein 2 homolog 1; Cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cell division protein kinase 1-A; CG5363; CG5363 gene product from transcript CG5363-RA; CG5363-PA; cyclin dependent kinase; cyclin dependent kinase 1; cyclin-dependent kinase; Cyclin-dependent kinase 1; cyclin-dependent kinase 1 S homeolog; Cyclin-dependent kinase 1-A; D2366; Dcdc2; dCdk1; DKFZp686L20222; Dm cdc2; Dmcdc2; DmCdk1; Dmel\CG5363; Dmel_CG5363; DNA polymerase delta catalytic subunit; DNA polymerase III; DNA-directed DNA polymerase delta POL3; group 4; HPR6; KCIP-1; l(2)31Eh; maturation-promoting factor; MGC111195; MGC53829; MPF; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; p34 protein kinase 1; p34; P34CDC2; POL3; Protein 1054; protein 2; PSTAIR; TEX1; xcdc2; XELAEV_18036802mg; YDL102W
• Gene Symbols: CDK1
• Host Species: Mouse
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12534, 54237, 983
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ