Invitrogen™ Cdc27 Monoclonal Antibody (AF3.1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA124749

Catalog No. PIMA124749

Description

Recommended positive controls: HeLa nuclear extract. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: Cdc27
• Classification: Monoclonal
• Concentration: 2.3 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: A2A6Q5, A7Z061, P30260, Q4V8A2
• Gene Symbols: CDC27, cdc27.S
• Immunogen: Synthetic peptide, conjugated to KLH, corresponding to residues 814-823 of human Cdc27.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Bovine, Frog
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: AF3.1
• Conjugate: Unconjugated
• Gene: CDC27
• Gene Alias: AI452358; AN CDC27Hs; ANAPC3; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; BC023187; cdc27; CDC27 homolog; cdc27.S; CDC27Hs; cell division cycle 27; cell division cycle 27 homolog; cell division cycle 27 homolog (S. cerevisiae); cell division cycle 27 S homeolog; cell division cycle protein 27 homolog; D0S1430E; D17S978E; HNUC; H-NUC; hypothetical protein; NUC2; nuc2 homolog; XELAEV_18046079mg
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 217232, 360643, 443994, 490924, 540660, 996
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid