Invitrogen™ Cdc27 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA132473

Catalog No. PIPA132473

Description

Recommended positive controls: Hela whole cell lysate.

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: Cdc27
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CDC27
• Gene Alias: AI452358; AN CDC27Hs; ANAPC3; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; BC023187; cdc27; CDC27 homolog; cdc27.S; CDC27Hs; cell division cycle 27; cell division cycle 27 homolog; cell division cycle 27 homolog (S. cerevisiae); cell division cycle 27 S homeolog; cell division cycle protein 27 homolog; D0S1430E; D17S978E; HNUC; H-NUC; hypothetical protein; NUC2; nuc2 homolog; XELAEV_18046079mg
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 996
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 1.95 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P30260
• Gene Symbols: CDC27
• Immunogen: synthetic peptide NINDSLEIT, corresponding to Internal sequence amino acids 422-430 of Human Cdc27.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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