CDC27Rabbit anti-Human, Polyclonal, Rockland™

Catalog No.	Quantity
50-199-1825	100 μg

Catalog No. 50-199-1825 Supplier Rockland Immunochemicals Supplier No. 600401868

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity purified antibody is directed against human Cdc27 protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, chicken, rat and dog based on 100% homology for the immunogen sequence. Expect cross reactivity with cdc27 from mouse as only a single amino acid residue change is found for the immunogen sequence. Cross reactivity with cdc27 homologues from other sources has not been determined.

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: CDC27
• Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: CDC27
• Gene Accession No.: P30260
• Gene Alias: AI452358; AN CDC27Hs; ANAPC3; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; BC023187; BOS_19285; CDC27; CDC27 homolog; cdc27.S; CDC27Hs; cell division cycle 27; cell division cycle 27 homolog; cell division cycle 27 homolog (S. cerevisiae); cell division cycle 27 S homeolog; Cell division cycle protein 27 homolog; D0S1430E; D17S978E; HNUC; H-NUC; NUC2; nuc2 homolog; XELAEV_18046079mg
• Gene Symbols: CDC27
• Host Species: Rabbit
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 996
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid