Invitrogen™ CDC34 Polyclonal Antibody

Catalog No.	Quantity
PIPA5120918	100 μL

Catalog No. PIPA5120918 Supplier Invitrogen™ Supplier No. PA5120918

Description

Rabbit Polyclonal Antibody

Positive test controls include: HeLa, Jurkat, C6, SH-SY5Y, Mouse testis. The target is usually found in the following locations: Cytoplasm, Nucleus. Immunogen sequence: KWKESKGKDR EYTDIIRKQV LGTKVDAERD GVKVPTTLAE YCVKTKAPAP DEGSDLFYDD YYEDGEVEEE ADSCFGDDED DSGTEES.

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF (FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF (SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

Specifications

• Antigen: CDC34
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1.28 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.3
• Gene: CDC34
• Gene Accession No.: P49427, Q8CFI2
• Gene Alias: (E3-independent) E2 ubiquitin-conjugating enzyme R1; AI327276; CDC34; cell division cycle 34; cell division cycle 34 homolog; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; serine protease; UBC3; UBCH3; UBE2R1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme Cdc34; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
• Gene Symbols: CDC34
• Host Species: Rabbit
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 150-236 of human CDC34 (NP_004350.1).
• Purification Method: Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 216150, 299602, 997
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG