Invitrogen™ Cdc34 Recombinant Rabbit Monoclonal Antibody (JJ086-07)

Catalog No.	Quantity
PIMA532420	100 μL

Catalog No. PIMA532420 Supplier Invitrogen™ Supplier No. MA532420

Description

Rabbit Recombinant Monoclonal Antibody

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF (FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF (SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

Specifications

• Antigen: Cdc34
• Applications: Flow Cytometry, Western Blot, Immunocytochemistry, Western Blot
• Classification: Recombinant Monoclonal
• Clone: JJ086-07
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4
• Gene: CDC34
• Gene Accession No.: P49427, Q8CFI2
• Gene Alias: (E3-independent) E2 ubiquitin-conjugating enzyme R1; AI327276; CDC34; cell division cycle 34; cell division cycle 34 homolog; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; serine protease; UBC3; UBCH3; UBE2R1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme Cdc34; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
• Gene Symbols: CDC34
• Host Species: Rabbit
• Immunogen: Recombinant protein within Human Cdc34 aa 1-236.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 216150, 299602, 997
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG