Cdc42, Mouse anti-Mouse, Yeast, Human, Clone: 28-10, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABN248525UL

Catalog No. MABN248525

Description

Anti-Cdc42, clone 28-10, Cat. No. MABN2485, is a highly specific mouse monoclonal antibody that targets Cell division control protein 42 (Cdc42) and has been tested for use in Immunocytochemistry and Western Blotting.

Cell division control protein 42 (UniProt: P19073; also known as Cdc42, Suppressor of RHO3 protein 2) is encoded by the CDC42 (also known as SRO2, TLR229C, L8083.13) gene (Gene ID: 850930) in yeast (Saccharomyces cerevisiae). Cdc42 is a member of the Rho GTPase family that regulates multiple signaling pathways involved in actomyosin organization, cell proliferation, and maintenance of cell polarity in most eukaryotic cell types. Cdc42 signaling process involves guanine nucleotide exchange factors (GEFs), guanine nucleotide dissociation inhibitors (GDIs), and GTPase-activating proteins (GAPs). In yeast, generation and maintenance of robust Cdc42 polarity promotes membrane expansion during bud formation and it has been shown that Cdc42 is dynamically maintained at the polarity cap through its continuous cycling between the polarity cap and internal pools. Cdc42 is highly polarized during bud emergence and is specifically required for exocytosis at this stage. Cdc42 is synthesized with a propeptide region (aa 189-191), which is cleaved off in the mature form. The mature form contains three nucleotide (GTP)-binding sites. (Ref.: Wu, H., and Brennwald, P. (2010) Mol. Cell Biol. 30(21); 5207-5217).

Specifications

• Antigen: Cdc42
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: CDC42;SRO2;TLR229C;L8083.13
• Immunogen: GST-tagged full-length recombinant yeast Cdc42.
• Quantity: 25 μL
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_013330
• Target Species: Mouse, Human, Yeast
• Form: Purified

• Applications: Immunocytochemistry, Western Blot
• Clone: 28-10
• Dilution: Western Blotting Analysis: A representative lot detected Cdc42 in Western Blotting applications (Sullivan, C.S., et. al. (2016). J Biol Chem. 291(51):26262-26272; Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).Immunocytochemistry Analysis: A representative lot detected Cdc42 in Immunocytochemistry applications (Wu, H., et. al. (2010). Mol Cell Biol. 30(21):5207-17).
• Gene Alias: Cell division control protein 42;Suppressor of RHO3 protein 2
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 28-10 specifically detects Cell division control protein 42 (Cdc42).
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG1 κ