Invitrogen™ CDCA2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA556322

Catalog No. PIPA556322

Description

Immunogen sequence: IKCERKDDFL GAAEGKLQCN RLMPNSQKDC HCLGDVLIEN TKESKSQSED LGRKPMESSS VVSCRDRKDR RRSMCYSDGR SLHLEKNGNH TPSS Highest antigen sequence identity to the following orthologs: Mouse - 44%, Rat - 46%.

Cell division cycle associated 2 (CDCA2, Repo-Man) is a cell-cycle protein that recruits protein phosphatase 1 (PP1) to mitotic chromatin at anaphase onset, which is essential for cell proliferation. Carboxy-terminal phosphorylation of CDCA2 at Ser893 by Aurora B inhibits the protein and leads to diffuse localization during prometaphase and metaphase. Dephosphorylation of CDCA2 by PP2A is necessary for CDCA2/PP1 complex reformation. The CDCA2/PP1 complex is required for chromatin binding and dephosphorylation of histone H3 at Thr3, Ser10, and Ser28. The CDCA2/PP1 complex is also involved in nuclear envelope reformation during mitotic exit for proper progression through the M/G1 transition. The interaction of CDCA2 with importin beta and Nup153, which is required for nuclear envelope formation, is negatively regulated by CDK phosphorylation of the amino-terminal domain of CDCA2. CDCA2 may play a role in DNA repair as the release of CDCA2 from chromatin at sites of DNA damage promotes the activation of DNA damage response. These results imply that the CDCA2/PP1 complex may play a part in cancer progression. Research studies indicate that CDCA2 may serve as a prognostic marker, as increased CDCA2 expression is seen in a number of cancers, including melanoma, neuroblastoma tumors, squamous cell carcinoma, and synovial sarcomas.

Specifications

• Antigen: CDCA2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CDCA2
• Gene Alias: 2610311M19Rik; AI586158; CDCA2; cell division cycle associated 2; cell division cycle-associated protein 2; FLJ25804; MGC129906; MGC129907; PPP1R81; protein phosphatase 1, regulatory subunit 81; recruits PP1 onto mitotic chromatin at anaphase protein; Repo-Man
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 157313
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry
• Concentration: 0.5 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q69YH5
• Gene Symbols: CDCA2
• Immunogen: Recombinant protein corresponding to Human CDCA2. Recombinant protein control fragment (Product #RP-93942).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG