MilliporeSigma™ CDK12, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABE1861

Catalog No. ABE1861MI

Description

Cyclin-dependent kinase 12 (EC 2.7.11.22; EC 2.7.11.23; UniProt Q9NYV4; also known as CDC2-related protein kinase 7, Cdc2-related kinase, arginine/serine-rich, Cell division cycle 2-related protein kinase 7, Cell division protein kinase 12, CrkRS, hCDK12) is encoded by the CDK12 (also known as CRK7, CRKRS, KIAA0904) gene (Gene ID 51755) in human. CDK12 belongs to the CMGC Ser/Thr protein kinase subfamily of cyclin-dependent kinases (CDKs). Originally identified and characterized as an RNA Polymerase II C-terminal domain (CTD) kinase, CDK12 is involved in the 3' end processing of the MYC and c-FOS mRNA transcripts. In addtion, CDK12/CyclinK complex is implicated in the maintenance of genomic stability through the regulation of DNA damage response genes and as a determinant of PARP1/2 inhibitor sensitivity in cancer treatment. Human CDK12 is uncharacteristically large with a molecular weight of ∽160kDa (most CDKs are about 50kDa)and contains large N- and C-terminal extensions flanking the kinase domain (a.a. 727-1020). These extensions contain multiple stretches of low complexity sequence and RS domains characteristic of splicing factors and regulators believed to mediate protein-protein interactions. As expected of RS domain-containing proteins, CDK12 is found in nuclear speckles representing storage sites for splicing factors.

Specifications

• Antigen: CDK12
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CDK12, CRK7, CRKRS, KIAA0904
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Immunogen sequence is present in all spliced isoforms of human, mouse, and rat CDK12 reported by UniProt (Q9NYV4, Q14AX6, and Q3MJK5).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

• Applications: Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: Q9NYV4
• Immunogen: Linear peptide corresponding to an N-terminal sequence of human CDK12.
• Quantity: 100 μg
• Research Discipline: Epigenetics & Nuclear Function
• Gene ID (Entrez): NP_057591
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.