CDK2 Monoclonal Antibody (5B12D1), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA549149

Catalog No. PIMA549149

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.

CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

Specifications

• Antigen: CDK2
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4MG trehalose and no preservative
• Gene Accession No.: P24941, P97377, Q63699
• Gene Symbols: CDK2
• Immunogen: E.coli-derived human Cdk2 recombinant protein (Position: E81-L298).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Clone: 5B12D1
• Conjugate: Unconjugated
• Gene: CDK2
• Gene Alias: A630093N05Rik; CDC28; CDC2A; cdc2-related protein kinase; CDK1; CDK2; CDKN2; Cell division protein kinase 2; cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33(CDK2); p33CDK2; p34 protein kinase
• Host Species: Mouse
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1017, 12566, 362817
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Isotype: IgG2a