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Invitrogen™ CDK2 Monoclonal Antibody (SP80)
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Catalog No. PIMA516346
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500 μL
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PIMA516346 500 μL
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Catalog No. PIMA516346 Supplier Invitrogen™ Supplier No. MA516346
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Description

Rabbit Monoclonal Antibody

Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue (IHC) or Hela lysate (WB).

CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CDK2
Applications Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
Classification Monoclonal
Clone SP80
Concentration 0.031 mg/mL
Conjugate Unconjugated
Formulation PBS with 1% BSA and 0.1% sodium azide; pH 7.6
Gene CDK2
Gene Accession No. P24941, P97377, Q63699
Gene Alias A630093N05Rik; CDC28; CDC2A; cdc2-related protein kinase; CDK1; CDK2; CDKN2; Cell division protein kinase 2; cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33(CDK2); p33CDK2; p34 protein kinase
Gene Symbols CDK2
Host Species Rabbit
Immunogen Synthetic peptide corresponding to C-terminus of human CDK2 protein.
Purification Method Protein A/G
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 1017, 12566, 362817
Target Species Human, Mouse, Rat
Content And Storage 4°C, do not freeze
Product Type Antibody
Form Liquid
Isotype IgG
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