Invitrogen™ Human CDK3/cyclin E1, GST Tag Recombinant Protein

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Protein Form: Recombinant The gene accession number for the subunits CDK3 and Cyclin E1 are NP_001249.1 and NP_001229.1.

CDK3/CyclinE1is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis. The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported.

Specifications

• Accession Number: Q00526
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM tris HCl with 0.1 mM EDTA, 0.1 mM EGTA, 0.1 mM PMSF, 0.25 mM DTT, 150 mM NaCl, 25% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 1018
• Molecular Weight (g/mol): 61.3-73.4 kDa
• Name: Human CDK3/cyclin E1, GST Tag
• Quantity: 10 μg
• Gene Alias: 2310050O10Rik; Cdk3; Cdk3-ps; CDKN3; Cell division protein kinase 3; cyclin dependent kinase 3; cyclin-dependent kinase 3; cyclin-dependent kinase 3, pseudogene
• Common Name: CDK3
• Protein Length: 1-305
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Baculovirus
• Form: Liquid