CDKN1A Mouse anti-Human, Clone: SPM306, Abnova™

Catalog No.	Quantity
89-933-929	100 μg

Catalog No. 89-933-929 Supplier Abnova Corporation Supplier No. MAB13039

Description

Mouse monoclonal antibody raised against full length recombinant human CDKN1A.

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Specifications

• Antigen: CDKN1A
• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (PFA fixed), Western Blot
• Classification: Monoclonal
• Clone: SPM306
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against full length recombinant human CDKN1A.
• Dilution: Flow Cytometry (0.5-1 ug/million cells in 0.1 mL) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL) Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the
• Formulation: In 10mM PBS.
• Gene Alias: CAP20/CDKN1/CIP1/MDA-6/P21/SDI1/WAF1/p21CIP1
• Gene Symbols: CDKN1A
• Host Species: Mouse
• Immunogen: Recombinant protein corresponding to full length of human CDKN1A.
• Purification Method: Protein A/G purification
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1026
• Test Specificity: This antibody is highly specific to CDKN1A and shows no cross-reaction with other closely related mitotic inhibitors.
• Target Species: Human
• Content And Storage: Store at -20 to -80°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid
• Isotype: IgG2a κ