CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247973A	Novus Biologicals NBP2479730.1MG	0.1 mg
NBP247973B	Novus Biologicals NBP2479730.2MG	0.2 mg

Description

CEACAM5/CD66e Monoclonal specifically detects CEACAM5/CD66e in Human, Monkey samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: CEACAM5/CD66e
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cellular Markers, Immunology
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 1048
• Immunogen: Recombinant full-length human CEA protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: C66/195
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 1 - 2 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Monkey
• Gene Alias: Carcinoembryonic antigen, carcinoembryonic antigen-related cell adhesion molecule 5, CD66e antigen, CEACD66e, DKFZp781M2392, Meconium antigen 100
• Gene Symbols: CEACAM5
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix