CEACAM5/CD66e Antibody (COL-1 + CEA31 + C66/261), Alexa Fluor™ 750, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234597AF750

Catalog No. NP23497A70

Description

CEACAM5/CD66e Monoclonal specifically detects CEACAM5/CD66e in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CEACAM5/CD66e
• Classification: Monoclonal
• Conjugate: Alexa Fluor 750
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: CEACAM5
• Immunogen: Human colon carcinoma extract (COL-1 & CEA31); Recombinant full-length human CEA protein (C66/261) (Uniprot: P06731)
• Quantity: 0.1 mL
• Gene ID (Entrez): 1048
• Target Species: Human
• Isotype: IgG1, IgG2a

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: COL-1 + CEA31 + C66/261
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Alias: Carcinoembryonic antigen, carcinoembryonic antigen-related cell adhesion molecule 5, CD66e antigen, CEACD66e, DKFZp781M2392, Meconium antigen 100
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Primary or Secondary: Primary
• Test Specificity: This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This monoclonal antibody does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbladder, urachus, salivary gland, ovary, and endocervix.
• Content And Storage: Store at 4°C in the dark.