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Invitrogen™ eBioscience™ Cell Proliferation Dye eFluor™ 450

Catalog No. 50246096
Encompass_Preferred
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Quantity:
4 x 500 μg
500 μg

A violet fluorescent dye that can be used to monitor individual cell divisions for up to 7 generations.

  • A violet fluorescent dye that can be used to monitor individual cell divisions
  • Dye binds to any cellular proteins containing primary amines, and as cells divide, the dye is distributed equally between daughter cells
  • They can be measured as successive halving of the fluorescence intensity of the dye - visualize up to 7 generations
  • Cells labeled with Cell Proliferation Dye eFluor™ 450 may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers
  • Cell Proliferation Dye eFluor™ 450 has a peak excitation of 409 nm and can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™), making it compatible with applications that utilize GFP

Additional Details

  • Each vial may be reconstituted to a stock concentration of 10 mM with 165 μL of anhydrous DMSO
  • Once reconstituted it should be used within 6 months and protected from light and stored at -20°C with dessicant
  • Avoid freeze-thawing
TRUSTED_SUSTAINABILITY

Specifications

Color Violet
Detection Method Fluorescence
For Use With (Application) Proliferation Assay
For Use With (Equipment) Flow Cytometer
Product Type Dye
Emission 405/450
Form Lyophilized powder
Product Line eBioscience
Quantity 500 μg

For Research Use Only. Not for use in diagnostic procedures.

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