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Molecular Probes™ CellROX™ Deep Red Flow Cytometry Assay Kit

Catalog No. C10491
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Description

CellROX™ Deep Red Flow Cytometry Assay Kit

The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).

View a selection guide for all CellROX™ Reagents and Kits.

The CellROX™ Deep Red Flow Cytometry Assay Kit features:

  • Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
  • Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
  • Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free media

    The CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Cell Type Mammalian Cells, Eukaryotic Cells
    Content And Storage Contains 1 vial CellROX™ Deep Red Reagent (25 μL), 1 vial SYTOX™ Blue Dead Cell Stain (100 μL), 2 vials N-acetylcysteine (10 mg per vial), 1 vial tert-butyl hydroperoxide (50 μL, 70% solution in water), and 1 vial DMSO (200 μL).

    Store kit at -5 to -30°C, protected from light. CellROX™ reagent is air sensitive.
    Detection Method Fluorescence
    For Use With (Application) Flow Cytometry
    For Use With (Equipment) Flow Cytometer
    Product Type Reagent
    Dye Type CellROX™ Deep Red Reagent
    Emission 644/665, 444/480
    Format Tube(s)
    Product Line CellROX
    Quantity 100 Assays
    Shipping Condition Wet Ice
    Solubility DMSO (Dimethylsulfoxide)
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    For Research Use Only. Not for use in diagnostic procedures.

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