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Thermo Scientific™ CellSensor™ SIE-bla THP-1 Cell Line

Catalog No. K1652B
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Description

The CellSensor™ SIE-bla THP-1 cell line contains a beta-lactamase reporter gene under control of the Sis Inducible Element (SIE), stably integrated into THP-1 cells.

The CellSensor™ SIE-bla THP-1 cell line contains a beta-lactamase reporter gene under control of the Sis Inducible Element (SIE), stably integrated into THP-1 cells. To construct this cell line, the SIE-bla construct was transduced into THP-1 cells by lentivirus. Flow cytometry was used to isolate cells responsive to Interferon Gamma (IFNγ). This cell line is validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as Z´ and EC50 concentrations for IFNγ. The CellSensor™ SIE-bla THP-1 cell line is responsive to IFNγ and can be used to analyze the JAK/STAT pathway using the GeneBLAzer™ Loading Substrates. Academic and non-profit customers, please inquire for special pricing.

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Specifications

Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Druggable Target Cytokines, Signaling Pathway
Product Line CellSensor
Product Type Cell Line
Quantity 1 vial

For Research Use Only. Not for use in diagnostic procedures.

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