Invitrogen™ CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers

Description

CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers

Like CFDA SE, CellTrace™ Oregon Green™ 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green™ 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green™ 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green™ 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8).

Specifications

• Content And Storage: Contains 20 vials of Oregon Green™ 488 carboxylic acid diacetate, SE (50 μg/vial). Store in freezer (-5 to -30°C) and protect from light.
• Detection Method: Fluorescence
• For Use With (Application): Cell Viability and Proliferation
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
• Product Type: Carboxy-DFFDA SE
• Dye Type: Oregon Green™ 488
• Emission: 488
• Form: Solid
• Product Line: CellTrace, Oregon Green
• Quantity: 20 x 50 μg
• Reagent Type: Cell Tracker Compounds, Cell Labeling Reagents
• Shipping Condition: Room Temperature
• Solubility: DMSO (Dimethylsulfoxide)

For Research Use Only. Not for use in diagnostic procedures.