MilliporeSigma™ CENP-C, Rabbit, Unlabeled, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABE1957

Catalog No. ABE1957

Description

Centromere protein C (UniProt Q03188; also known as CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7) is encoded by the CENPC (also known as CENPC1, ICEN7) gene (Gene ID 1060) in human. Kinetochores assemble on centromeres to bind spindle microtubules and mediate chromosome segregation during cell division. CENP-C is a subunit of constitutive centromere-associated network (CCAN) that acts as the centromere-kinetochore interface. CCAN is a large complex composed of at least 16 different centromeric proteins (CENPs). CENP-C plays an important role in kinetochore assembly downstream of CENP-A. The PEST domain in the N-terminal half of CENP-C interacts directly with the CCAN subcomplex CENP-HIKM (CENP-H, CENP-K, CENP-I, and CENP-M) and CENP-C depletion is shown to cause mislocalization of CENP-T and the CENP-HIKM complex subunits in HeLa cells.

Specifications

• Antigen: CENP-C
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CENPC, CENPC1, ICEN7
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This rabbit polyclonal antiserum targets the N-terminal region of human CENP-C present in both spliced isoforms reported by UniProt (Q03188). Specificity was demonstrated by a reducion of target band detection (Western blotting) and kinetochores staining (chromsome spreads fluorescence staining) following cellular CENP-C shRNA induction (Falk, S.J., et al. (2015). Science. 348(6235):699-703).
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• Applications: Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
• Gene Accession No.: Q03188
• Immunogen: GST-tagged recombinant human CENP-C N-terminal fragment.
• Quantity: 100 μL
• Research Discipline: Epigenetics & Nuclear Function
• Gene ID (Entrez): NP_001803
• Target Species: Human
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.